minimally processed data
~60 GB |
Aligned, normalized and compressed
~6 GB |
Initial processing in UCSF Chimera
# First convert deconvolved tif images to cmap file format, adding correct voxel size.
#cell18 march 10
vseries open '/PATH_TO_ACTIN_DATA_FOR_CELL18_MARCH10/r/*.am';
vseries save #0 '/PATH_TO_NEW_DATA_FOLDER/cell18_march10_actin.cmap' value float32 zeroMean true encloseVolume 500 normalizeLevel 100;
vseries close #0
vseries open '/PATH_TO_ACTIN_DATA_FOR_CELL18_MARCH10/g/*.am';
vseries save #1 '/PATH_TO_NEW_DATA_FOLDER/cell18_march10_membrane.cmap' value float32 zeroMean true encloseVolume 500 normalizeLevel 100;
vseries close #1
# First convert deconvolved tif images to cmap file format, adding correct voxel size.
#cell18 march 10
vseries open '/PATH_TO_ACTIN_DATA_FOR_CELL18_MARCH10/r/*.am';
vseries save #0 '/PATH_TO_NEW_DATA_FOLDER/cell18_march10_actin.cmap' value float32 zeroMean true encloseVolume 500 normalizeLevel 100;
vseries close #0
vseries open '/PATH_TO_ACTIN_DATA_FOR_CELL18_MARCH10/g/*.am';
vseries save #1 '/PATH_TO_NEW_DATA_FOLDER/cell18_march10_membrane.cmap' value float32 zeroMean true encloseVolume 500 normalizeLevel 100;
vseries close #1